Facts About attune flow cytometer Revealed

Facts About attune flow cytometer Revealed

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Facts-enriched characterization of cell populations. Morphological information and facts from visuals can insert to the richness of flow cytometry info. One example is, the determine displays an usually traditional apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Each individual population to reveal morphologically distinct features. These insights could not are already acquired from multiplex staining on your own.

As samples are obtained on the Attune CytPix Flow Cytometer, the superior-pace brightfield camera captures and suppliers photographs of detected situations in a charge up to 6,000 photos for every next, depending on the flow level and picture size. For bigger flexibility, Attune Cytometric Program enables you to adjust the impression seize frequency as necessary and choose a selected gate for picture capture, if needed.

(B) Uncooked 264.seven macrophages tend to be annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The volume of singlets obtainable for Evaluation will increase as soon as the consumer trains and employs a far more precise product.

Uncover new insights into your experimental details by visualizing populations making use of mixed fluorescence and morphology data. Our computer software interface wizards help downsampling, concatenation (merged documents) and dimensionality tools that happen to be created in the computer software for an intuitive, streamlined info Examination expertise.

*Pricing is indicative only and is also subject to change. Be sure to Get in touch with your Agilent agent with any questions. The data set forth herein will not be an offer or possibly a commitment, equally of that are issue to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Economical Methods is offered in picked international locations.

Listed here, a skilled user has gated singlets confidently. Just after evaluating the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate incorporates over four% aggregates.

Pair your Attune flow cytometer by having an autosampler for amplified workflow efficiency, and add robotic integration for maximum throughput.

Our scientists will structure panels for you personally as over and over you will need—totally free, no product or service order necessary.

Early signs of this contamination had in the beginning been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also helps you to use morphological features to discover attention-grabbing subpopulations that may not be evident from flow cytometry details on your own.

Consumers can see party images in true-time to substantiate sample high quality, enhance protocols, and file the morphology of each cell populace discovered for analysis. Imaging seize also benefits from acoustic focusing, which helps to position the cells so that a sharp, centered image is attained.

AI-pushed automated image Examination With all the Attune Cytometric Program interprets function capabilities into unique morphology parameters that may be coupled with typical fluorescence and scatter parameters to expose really reproducible inhabitants alterations and exceptional subsets.

Software Take note: Automated image analysis minimizes consumer-to-person variability in flow cytometry gating tactics

Leverage the power of Highly developed know-how and innovation to accelerate your discoveries. Rely on in the Attune Flow Cytometer household to deliver the trustworthiness, pace, and precision that set your work aside.

No clean/no lyse leukocyte analysis with imaging. Cells had been acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Skilled UX/UI enables consumers to practice AI types for expanded applications. (A) The “Educate Model” characteristic delivers annotation equipment and authentic-time product teaching precision.

At the heart of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which makes use of sound waves to meticulously align cells throughout the flow cell. This modern technique surpasses classic hydrodynamic solutions, causing more quickly processing speeds, Remarkable information top quality, and improved sample throughput.

The Attune Xenith cytometer is really a high-parameter spectral analyzer, giving each spectral unmixing and traditional flow abilities for detailed and flexible mobile Investigation.

The Attune CytPix Flow Cytometer makes it possible for customers to visualize samples all through acquisition, validate the accuracy of gating tactics, and help success with reliable documentation for publication applications.

Here, we clearly show that by gating methods making use of picture-centered quantitative parameters can distinguish interacting cells from coincident gatherings website to extra precisely evaluate interacting cells.